GoldStar Probe Mixture is a premixed system specially designed for real-time fluorescence quantitative PCR by probe method (TaqMan, Molecular Beacon, etc.), the concentration is 2x, Consists of GoldStar Taq DNA Polymerase, PCR Buffer, dNTPs and Mg2+, which is easy to operate. It is mainly used for the detection of genomic DNA target sequence and RNA cDNA target sequence after reverse transcription, such as gene expression analysis, copy number analysis, SNP genotype analysis, etc. It is suitable for fluorescence quantification by different types of probes. GoldStar Taq DNA Polymerase is a new, highly efficient and chemically modified hot start enzyme. It has no polymerase activity at room temperature, effectively avoiding the non-specific amplification caused by the non-specific binding of primer and template or primer dimer at room temperature. The enzyme is activated by incubation at 95℃ for 10 minutes. The combination of the unique PCR buffer system and the hot start enzyme significantly improves the amplification efficiency of PCR, with stronger fluorescence signal and higher sensitivity, and can detect single copy of the template. With this product, a wider linear range can be obtained and the target gene quantification is more accurate. Suitable for all fluorescent quantitative PCR machines that do not require ROX as a correction dye. ROX dye is used to correct the fluorescence signal error generated between the holes of the quantitative PCR instrument. It is generally used in ABI, Stratagene and other companies' Real Time PCR amplifiers. The excitation optical system of different instruments is different, so the concentration of ROX dye must be matched with the corresponding fluorescence quantitative PCR instrument. Instruments that do not require ROX correction (CW0932) : Roche LightCycler 480, Roche LightCyler 96, Bio-rad iCyler iQ, iQ5, CFX96 and other instruments that require Low ROX correction (CW2625) : ABI Prism7500/7500 Fast, QuantStudio 3 System, QuantStudio5 System,QuantStudio 6 Flex System, QuantStudio 7 Flex System, ViiA 7 system,Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000, etc. Instruments requiring High ROX correction (CW2626) :ABI Prism7000/730017700/7900, Eppendorf, ABI Step One/Step One Plus, etc.