This product is a premixed system composed of GoldStar DNA Polymerase, Mg²⁺, dNTPs, and PCR stabilizers and enhancers at a concentration of 2x. This product has the advantages of simple and quick operation, high sensitivity, high specificity and good stability, which decrease human error and contamination maximumly. The GoldStar Best DNA polymerase has 5’-3’ DNA polymerase activity, 5’-3’ exonuclease activity and 3’-5’ exonuclease activity. Compared with GoldStar DNA Polymerase, this polymerase has higher amplification efficiency and lower mismatch rate under normal PCR conditions. The chemical-modified enzyme does not have polymerase activity at room temperature, thus effectively avoiding non-specific amplification. The polymerase must be activated by incubation at 95 °C for 10 minutes. The optimized buffer system maximizes the effect of the enzyme and achieves high fidelity, high specificity, high amplification efficiency, and high sensitivity amplification of the target fragment. This product does not contain dyes, and an appropriate amount of sample loading buffer should be added for electrophoresis. Most of the amplified PCR products have an "A" base attached to the 3' end, and therefore can be directly used for T/A cloning. It is mainly suitable conventional PCR reactions and experiments such as gene cloning that require high fidelity.