This product is a premixed system composed of Flash HotStart DNA Polymerase,Mg2+, dNTPs, PCR stabilizers and enhancers at a concentration of 2×. The Flash HotStart DNA Polymerase is featured with fast extension rate (5-15 sec/kb) and fast HotStart, which significantly shorten the duration of PCR. Meanwhile, it efficiently removes the non-specification amplification and enables high yield of PCR with high fidelity. This product has been added with dye (blue), and electrophoresis gel detection can be performed directly after the PCR. Most of the amplified PCR products have an "A" base attached to the 3' ends, and therefore can be directly used for T/A cloning. It provides a broad application for all conventional PCR reactions, such as gene cloning and genotyping.