This product is a first-strand cDNA synthesis kit designed for the first step of two-step RT-PCR. The HiFiScript reverse transcriptase used in the kit is a novel high-efficiency reverse transcriptase derived from M-MLV. The novel mutation site greatly enhances the transcriptional activity of the enzyme. The efficiency and yield of cDNA first-strand synthesis are higher, and this kit can be used with as little as pg level of total RNA or mRNA. The point mutation eliminates RNase H activity, improves the elongation ability of the enzyme, and effectively improves the affinity of the reverse transcriptase to the RNA template, resulting in a first strand cDNA of up to 12 kb. Optimized RT Buffers allow a wider range of applications for reverse transcriptase and are highly compatible with subsequent PCR and quantitative PCR experiments. The kit is equipped with all reverse transcription reagents and is easy to use. It is suitable for the synthesis of first-strand cDNA and subsequent RT-PCR, RT-qPCR, and construction of full-length cDNA libraries.
Nucleic Acid Release Reagent