This product is a kit for reverse transcription after removal of genomic DNA. The kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription reagents contain components that inhibit gDNA Remover, the sample processed by gDNA remover can be directly used for reverse transcription reaction to synthesize cDNA.
This kit contains a novel high-performance reverse transcriptase HiFiScript. 5×HifiScript RT MasterMix contains all the components needed for reverse transcription. The novel mutation site greatly enhances the transcriptional activity of the enzyme. The efficiency and yield of cDNA first-strand synthesis are higher, and the first strand of cDNA can be synthesized using pg total RNA or mRNA. If the cDNA is used for downstream qPCR, the reverse transcription reaction can be completed in 15 minutes. This kit is suitable for the synthesis of first-strand cDNA and subsequent RT-PCR, RT-qPCR, and construction of full-length cDNA libraries.