HiFi II M-MLV (H-) Reverse Transcriptase (Glycerol-free) is a glycerol-free version of HiFi II M-MLV(H-)Reverse Transcriptase (CW0743). It is a reverse transcription enzyme in which the mutant M-MLV gene is recombined and expressed by E. coli engineering bacteria. The enzyme cancatalyze the polymerization of complementary DNA using RNA or DNA: RNA hybrid chain as template. The mutated HiFi II M-MLV(H-) reverse transcriptase lacks RNase H activity, reducing RNA degradation during reverse transcription and making it easier to obtain full-length cDNA. HiFi II M-MLV reverse transcriptase can synthesize the first strand cDNA at 55 °C, providing higher specificity and stability, and can synthesize up to 12 kb cDNA with high cDNA yield. It is suitable for synthesis of first strand cDNA, RT-PCR, RT-qPCR, preparation of full-length cDNA library and the application of freeze-dried RT-PCR products. This product does not contain freeze-dried forming components, and it can be customized when applied to freeze-dried products.