Bst 3.0 Enzyme Mix is a mixture of Bst DNA polymerase and high-temperature reverse transcriptase. Bst DNA polymerase is a recombinase expressed and purified by E. coli with partial point mutations based on the original sequence. This product has stronger 5'→3' DNA polymerase activity, strand displacement activity, reverse transcription activity, and no 5'→3' exonuclease activity. High-temperature resistant reverse transcriptase is a new enzyme modified by genetic engineering. It has fast cDNA synthesis speed, and its thermal stability is greatly improved. It can tolerate reaction temperatures up to 60℃, which is suitable for reverse transcription reaction of RNA templates with complex secondary structure. Bst 3.0 Enzyme Mix can be applied to isothermal amplification reactions using RNA or DNA as templates (LAMP/RT-LAMP).