SuperFastStar DNA Polymerase (Glycerol-free) is a mixture of glycerol-free anti-Taq enzyme monoclonal antibody and Taq DNA Polymerase for Hot Start PCR. When using Taq enzyme antibody for PCR amplification, the combination of Taq enzyme antibody and Taq enzyme inhibits the activity of DNA polymerase before high temperature denaturation, which could effectively inhibit the non-specific annealing of primer and the non-specific amplification caused by primer dimer at low temperature.

The Taq enzyme antibody is denatured in the initial DNA denaturation step of PCR reaction, and the polymerase activity is restored to achieve the hot start effect. The use of this product does not require special inactivation of Taq enzyme antibodies, and can be used under conventional PCR reaction conditions.SuperFastStar DNA Polymerase (Glycerol-free) has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity, and no 3′-5′ exonuclease activity. Enzyme extension rate is 2 kb/min, and can amplify fragments up to 5 kb. The amplified PCR product has an " A " base attached to the 3′ end and can therefore be used directly for T/A cloning. The blocking rate of polymerase activity at a temperature of 55 °C and below is more than 95%, and DNA polymerase activity can be restored by heating at 95 °C for 5 s. This product has the characteristics of fast extension speed and high amplification efficiency, and is mainly suitable for lyophilization of single enzyme, multiplex amplification and DNA sequencing.