This kit adopts the new silicon matrix membrane technology and reagent formulation, through the unique centrifugal adsorption column can be quickly combined with the DNA-washing-elution step can be recovered from ordinary or low melting point agarose gel purification of DNA fragments of 100bp-10kb, the speed of lysis, high recovery rate. The lysate contains a pH indicator, which can be used to determine whether the lysate recovery is optimal according to the color. Each adsorption column can adsorb up to 10μg of DNA while effectively removing impurities.