Uracil-N-glycosylase (UNGase), also known as uracil DNA glycosylase (UDGase), is a recombinant enzyme expressed and purified by E. coli. The protein has a molecular weight of 25 kD and can catalyze uracil-containing Single- and double-stranded DNA releases free uracil and is inactive to RNA, and is mainly used to prevent contamination of PCR amplification products. The mechanism of action of the enzyme is as follows: in the PCR reaction, dUTP is used instead of dTTP, and all Ts in the amplified product fragments are replaced by U, forming a PCR amplification product containing dU bases. UNG enzyme can selectively break the glycosidic bond of U base in single-stranded and double-stranded DNA, degrade the DNA containing U in the reaction system, effectively eliminate the residual contamination of PCR products, and greatly reduce false positives caused by contamination of amplification products, thereby ensuring specificity and accuracy of amplification.
Nucleic Acid Release Reagent