This product is a chemically modified hot-start high-fidelity polymerase. The polymerase has 5'-3' DNA polymerase activity, 5'-3' exonuclease activity and 3'-5' exonuclease activity. Under normal PCR conditions, compared with GoldStar DNA Polymerase, it has higher amplification efficiency excellent performance with high and low mismatch rate. The chemically modified enzyme has no polymerase activity at room temperature, which can effectively avoid non-specific amplification caused by the non-specific binding of primer and template or primer dimer under normal temperature conditions. The activation of the enzyme must be incubated at 95°C for 10 minutes his which can be integrated into existing PCR thermal cycling programs. The optimized buffer system maximizes the effect of the enzyme to achieve high fidelity, high specificity, high amplification efficiency, and high sensitivity amplification of the target fragment. Most of the PCR products amplified with this product have an "A" base attached to the 3' end, so they can be directly used for T/A cloning. This product is used in conventional PCR, RT-PCR and multiplex PCR,especially for PCR with high requirements on specificity, fidelity and amplification efficiency.
Nucleic Acid Release Reagent